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Oxford Instruments 3d imaris filaments
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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Oxford Instruments 3d imaris filament tracer
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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Markforged Inc 3d printer with multiple filaments
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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Amira Pharmaceuticals 3d filament editor
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
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Image Search Results


A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of 3D morphometric reconstructions by using Imaris software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.

Journal: Communications Biology

Article Title: JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis

doi: 10.1038/s42003-025-08208-3

Figure Lengend Snippet: A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of 3D morphometric reconstructions by using Imaris software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.

Article Snippet: For a schematic illustration of the dendritic evaluation parameters, see Supplementary Fig. . For examples of Imaris-based morphology tracings, see the 2D representations of 3D Imaris filaments with their branch and terminal points shown in Supplementary Figs. and .

Techniques: Staining, Transfection, Expressing, Marker, Software